5th Central and Eastern European Proteomic Conference

SCIENTIFIC PROGRAM

Day 1 – Monday, September 19th 2011

13:00 – 17:00
Registration / Exhibition Setup
17:00 – 17:30
Welcome on behalf of Czech Proteomic Society and Central and Eastern European Conference board (Karel Bezouška, Suresh Jivan Gadher, Hana Kovářová)
17:30 – 19:00
Opening Session
(Plenary Talks)
Chair: Hana Kovářová, Suresh Jivan Gadher
 
17:30 – 18:15
Peter R. Jungblut
Max Planck Institute for Infection Biology, Core Facility Protein Analysis, Berlin, Germany
The Protein species concept: molecular basis of the search for biomarkers
18:15 – 19:00
Javier Munoz1, Teck Yew Low1, Adja Zoumaro1, Marco Benevento1, Daniel E. Stange2, Shabaz Mohammed1, Yee Jiun Kok3, Marc van de Wetering2, Andre Choo3, Hans Clevers2, Albert J. R. Heck1
1 Biomolecular Mass Spectrometry and Proteomics Group, Bijvoet Center for Biomolecular Research , Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands and Netherlands Proteomics Center;
2 Hubrecht Institute-KNAW (Royal Netherlands Academy of Arts and Sciences) and University Medical Center Utrecht, Uppsalalaan 8, 3584 CT Utrecht, Netherlands;
3 Stem Cell Group, Bioprocessing Technology Institute, Biopolis Way 20, Singapore
UNRAVELING KEY MOLECULAR COMPONENTS IN STEM CELL BIOLOGY WITH MASS SPECTROMETRY BASED PROTEOMICS
19:00
WELCOME RECEPTION

Day 2 – Tuesday, September 20th 2011

09:00 – 09:15
Welcome (Karel Bezouška, Suresh Jivan Gadher, Hana Kovářová)
09:15 – 10:45
Session 1: Proteomic Diversity and Related Fields - Stem Cell Proteomics and Proteomics Clinical Applications
(Plenary Talks)
Chair: Hana Kovářová, Suresh Jivan Gadher
 
09:15 – 10:00
Thorsten Müller1, Andreas Schrötter1, Frederic Brosseron1, Helmut E. Meyer2, Stefan Helling1, Katrin Marcus1
1 Dep. Functional Proteomics, Medizinisches Proteom-Center, Ruhr-University Bochum
2 Dep. Medical Proteomics/Bioanalytics, Medizinisches Proteom-Center, Ruhr-University Bochum, Germany
Biomarker discovery using proteomics – Application in neurodegenerative diseases
10:00 – 10:45
Ravi Sirdeshmukh
Institute of Bioinformatics, ITPL, Bangalore 560 066, India
Advances in Glioma Proteomics – New Technologies and Approaches
10:45 11:15
Coffee / Refreshments / Trade Exhibition
11:15 – 12:55
Session 2: Proteomic Diversity and Related Fields - Stem Cell Proteomics and Proteomics Clinical Applications
(Selected Talks)
Chair: Jiří Petrák
 
11:15 – 11:35
Jiřina Tylečková1, Damaris Bausch-Fluck2, Silvia Marsala3, Martin Marsala3, Hana Kovářová1, Bernd Wollscheid2
1 Institute of Animal Physiology and Genetics AS CR, v.v.i., Libechov, Czech Republic,
2 Institute of Molecular Systems Biology, ETH Zurich, Switzerland,
3 Anesthesiology Research Laboratory, UCSD San Diego, USA
THE NEURAL PROGENITOR SURFACEOME
11:35 – 11:55
Rita Hrabáková1, Madhu Kollareddy2, Kateřina Mairychová1, Petr Halada3, Marián Hajdúch2, Hana Kovářová1
1 Institute of Animal Physiology and Genetics, AS CR, v.v.i., Department of Reproductive and Developmental Biology, Libechov, Czech Republic
2 Institute of Molecular and Translational Medicine, Laboratory of Experimental Medicine, Palacky University and University Hospital in Olomouc, Faculty of Medicine and Dentistry, Olomouc, Czech Republic
3 Institute of Microbiology AS CR, v.v.i., Laboratory of Molecular Structure Characterisation, Prague, Czech Republic
Cancer cell resistance to AURK-directed therapy: implications for anti-cancer strategies
11:55 –12:15
Tadashi Kondo
Division of Pharmacoproteomics, National Cancer Center Research Institute, 5-1-1 Tsukiji, Chuo-ku, Tokyo 104-0045, Japan
Cancer Proteomics for Biomarker Development toward Personalized Medicine
12:15 – 12:35
Vojtech Tambor, Juraj Lenco, Marian Kacerovsky, Jiri Stulik
Institute of Molecular Pathology, University of Defence, Hradec Králové
Deep proteome characterization as a tool for identification of novel intraamniotic infection and inflammation biomarkers in preterm birth patients
12:35 – 12:55
A. Resemann, W. Evers, E. Belau, O. Drews, S. Pengelley, W. Jabs, D. Suckau
BRUKER DALTONIK GMBH, Bremen, Germany
SUBTRACTIVE LABEL-FREE QUANTIFICATION OF PHOSPHORYLATION SITES IN KAPCA_HUMAN
12:55 – 14:30
Lunch / Poster Viewing
14:30 – 15:45
Session 3: Proteomic Diversity and Related Fields - Protein Post-Translational Modifications and Functions
(Plenary Talk, selected talk and sponsor talk)
Chair: Karel Bezouška
 
14:30 – 15:05
Olivér Ozohanics, Lilla Turiák, Ádám Lengyel, Károly Vékey, László Drahos
Chemical Research Center Hungarian Academy of Sciences
Glycosylation Patterns and Glycopeptide Biomarkers
15:05 – 15:25
Jiří Suttnar
Institute of Hematology and Blood Transfusion, Prague
The estimation and proteomics of S-nitroso proteins.
15:25 – 15:45
Volker Kruft
AB SCIEX, Darmstadt, Germany
Novel Qualitative and Quantitative Mass Spectrometry Workflows: Solutions for the Comprehensive Characterization of Complex Samples
15:45 – 16:15
Coffee / Refreshments / Trade Exhibition
16:15 – 17:50
Session 4: Proteomic Diversity and Related Fields - Microbial Proteomics and Bioprocessing
(Plenary Talk, selected talk and sponsor talk)
Chair: Günter Allmaier
 
16:15 – 16:50
Martina Marchetti-Deschmann1, Michaela Helmel1, Andreas Posch2, Christoph Herwig2 and Günter Allmaier1
1 Institute of Chemical Technologies and Analytics, Vienna University of Technology,
2 Institute of Chemical Engineering, Vienna University of Technology
Monitoring the fermentation process of Penicillium chrysogenum by combining a gel based proteomics approach and intact cell mass spectrometry
16:50 – 17:10
Fíla, J.1,2, Matros, A.3, Čapková, V.1, Honys, D.1,2, Mock, H.-P.3
1 Laboratory of Pollen Biology, Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Rozvojová 263, 165 02 Praha 6, Czech Republic;
2 Department of Plant Experimental Biology, Faculty of Science, Charles University in Prague, Viničná 5, 128 44 Praha 2, Czech Republic;
3 Applied Biochemistry Group, Department of Physiology and Cell Biology, Leibniz Institute of Plant Genetic and Crop Plant Research, Corrensstraße 3, 06466 Gatersleben, Germany
Revealing phosphoproteins playing role in tobacco pollen activation in vitro
17:10 – 17:30
Katarína Klubicová1, Maksym Danchenko1,2, Ludovit Skultety3, Valentyna V. Berezhna2, Namik M. Rashydov2, Martin Hajduch1
1 Department of Reproduction and Developmental Biology, Institute of Plant Genetics and Biotechnology, Slovak Academy of Sciences, Nitra, Slovakia.
2 Department of Biophysics and Radiobiology, Institute of Cell Biology and Genetic Engineering, National Academy of Sciences of Ukraine, Kyiv, Ukraine.
3 Center for Molecular Medicine, BITCET, Institute of Virology, Slovak Academy of Sciences, Bratislava, Slovakia
Flax seed proteome in remediated, formerly radioactive, Chernobyl area
17:30 – 17:50
Andreas Wiesner
Advion BioSciences Ltd.
Current ESI-MS methods in protein analysis benefit from using a robotic platform for sample delivery and nanospray generation
18:00 – 19:30
Poster Session I (with refreshments)
(authors of posters No. T1 – T21 are requested to be present)
Chair: Lenka Hernychová
Please note that there will be a prize awarded to the best submitted poster!
19:30
CLOSE OF DAY TWO (Karel Bezouška, Suresh Jivan Gadher)
BAR AND MUSIC IN FOYER
20:30
SPEAKERS' DINNER

Day 3 – Wednesday, September 21st 2011

09:00 – 09:15
Welcome (Karel Bezouška, Suresh Jivan Gadher, Hana Kovářová)
09:15 – 10:40
Session 5: Proteomic Diversity and Related Fields - Plant Proteomics
(Plenary talk and Selected talks)
Chair: Jozef Šamaj
 
9:15 – 10:00
Wolfram Weckwerth
Department of Molecular Systems Biology, University of Vienna, Althanstrasse 14, 1090 Vienna, Austria
Green Systems Biology – from genotype to phenotype in ecosystems research and biotechnological applications
10:00 – 10:20
Jana Chalupová1, Michaela Sedlářová2, Marek Šebela1, Pavel Řehulka3, Michaela Helmel4, Martina Marchetti-Deschmann4, Günter Allmaier4
1 Centre of the Region Haná for Biotechnological and Agricultural Research - Department of Protein Biochemistry and Proteomics, Faculty of Science, Palacký University, Olomouc;
2 Department of Botany,Faculty of Science, Palacký University, Olomouc, Czech Republic;
3 Institute of Molecular Pathology, Faculty of Military Health Sciences, University of Defence, Hradec Králové, Czech Republic;
4 Institute of Chemical Technologies and Analytics, Vienna University of Technology, Vienna, Austria
Identification and biotyping of phytopathogens using MALDI-TOF mass spectrometry
10:20 – 10:40
A.M. Egorova, V.G. Yakovleva., I.A. Tarchevsky
Kazan Institute of Biochemistry and Biophysics of Kazan Science Centre of RAS, Kazan
Proteomic analysis of responses to salicylic acid and methyl jasmonate in pea roots
10:40 – 11:10
Coffee / Refreshments / Trade Exhibition
11:10 – 12:50
Session 6: Proteomic Diversity and Related Fields - Plant Proteomics
(Selected talks)
Chair: Marek Šebela
 
11:10 – 11:30
Tomáš Takáč, Tibor Pechan, Oľga Šamajová, Jozef Šamaj
Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science, Palacký University, Department of Cell Biology, Šlechtitelů 11, CZ-783 71 Olomouc, Czech Republic
Pharmaco-proteomic analysis of phosphatidylinositol 3/4-kinase inhibitor wortmannin in Arabidopsis
11:30 – 11:50
Petr Halada, Lucie Kohoutová, Hana Kourová, Anna Doskočilová, Jindřich Volc, Pavla Binarová
Institute of Microbiology AS CR, v.v.i., Laboratory of Molecular Structure Characterisation, Prague, Czech Republic
Protein interactions of gamma-tubulin reveal its multiple roles in acentrosomal plant cells
11:50 – 12:10
Černý Martin1, Jedelský Petr2, Novák Jan1, Schlosser Andreas3, Brzobohatý Břetislav1
Laboratory of Plant Molecular Biology, Mendel University in Brno & Institute of Biophysics AS CR, Brno
Proteomic profiling of heat- and cold-shock responses in Arabidopsis indicates crosstalk between temperature and cytokinin perception
12:10 – 12:30
Markéta Žďárská1,2, Pavlína Zatloukalová1,2, David Potěšil1,2, Ondrej Šedo1,2, Ondřej Novák3, Bedřich Pešek4, Eva Miklánková1, Jana Vašíčková1,2, Zbyněk Zdráhal1,2 and Jan Hejátko1,2
1 Department of Functional Genomics and Proteomics, Institute of Experimental Biology, Masaryk University, Kamenice 5/A2, 625 00 Brno, Czech Republic,
2 CEITEC - Central European Institute of Technology, Masaryk University, Kamenice 5/A2, 625 00 Brno, Czech Republic,
3 Laboratory of Growth Regulators, Faculty of Science, Palacký University and Institute of Experimental Botany ASCR, Šlechtitelů 11, 783 71 Olomouc, Czech Republic,
4 Institute of Experimental Botany, the Academy of Sciences of the Czech Republic, Rozvojová 263, 165 02 Prague, Czech Republic
Specificity of the Arabidopsis Shoot and Root Response to Cytokinins
12:30 – 12:50
Blanka Pekárová1,3,*, Tomáš Klumpler1,3,*, Olga Třísková2,3, Jakub Horák1,3, Séverine Jansen2,3, Radka Dopitová1,3, Petra Borkovcová1, Veronika Papoušková2,3, Eliška Nejedlá1,3, Vladimír Sklenář2,3, Jaromír Marek1,3, Lukáš Žídek2,3, Jan Hejátko1,3,4, Lubomír Janda1,3
1 Department of Functional Genomics and Proteomics;
2 National Centre for Biomolecular Research, Masaryk University, Kotlářská 2, CZ-61137 Brno, Czech Republic;
3 CEITEC-Central European Institute of Technology, Masaryk University, Žerotínovo nám. 9, CZ-60177 Brno, Czech Republic
4 Author for correspondence
* These authors contributed equally
Structure and binding specificity of the receiver domain of sensor histidine kinase CKI1 from Arabidopsis thaliana
12:50 – 14:00
Lunch / Poster Viewing / CEEPC Board Meeting
14:00 – 15:30
Poster Session II
(authors of posters No. W1 – W16 are requested to be present)
Chair: Lenka Hernychová
Please note that there will be a prize awarded to the best submitted poster!
15:30 – 16:00
Coffee / Refreshments / Trade Exhibition
16:00 – 17:50
Session 7: Structural and Functional Proteomics - Protein Interactions and Functions
(Plenary Talks)
Chair: Hana Kovářová, Suresh Jivan Gadher
 
16:00 – 16:45
Javier De Las Rivas
University of Salamanca, Salamanca, Spain
Exploring protein-protein interaction networks integrated with pathways to identify hub proteins and critical interactions.
16:45 – 17:30
Alexander Archakov
Institute of Biomedical Chemistry, RAMS, Moscow, Russia
The Three Bottlenecks in HPP Implementation
17:30 – 17:50
Lisitsa A., Poverennaya E., Filimonov A., Ponomarenko E.
Institute of Biomedical Chemistry, RAMS, Moscow, Russia
Creation of Knowledgebase using Gene-Centric Content Management System (CMS)
17:50
CLOSE OF DAY THREE (Karel Bezouška, Suresh Jivan Gadher)
19:30
CONFERFENCE DINNER AT U HAJKU CONFERENCE CENTRE

Day 4 – Thursday, September 22nd 2011

09:00 – 09:15
Welcome (Karel Bezouška, Suresh Jivan Gadher, Hana Kovářová)
9:15 – 10:50
Session 8: Novel Mass Spectrometry Methodologies and Outlook
(Plenary Talk and sponsor talks)
Chair: Karoly Vekey, Aleš Svatoš
 
9:15 – 10.00
Bernd Wollscheid
Institute of Molecular Systems Biology, Curych, Switzeland
Caught in the act: ligand-based receptor capturing (LRC) on living cells
10:00 – 10:30
Mark McDowall1, James Langridge1, Tim Riley2 and Scott Geromanos2
1 Waters MS Technologies Centre, Atlas Park, Manchester, United Kingdom
2 Waters Corporation, 34 Maple Street, Milford, MA, USA.
On-Line UPLC/Ion Mobility Separation/ToF MS for Qualitative and Quantitative Protein Profiling in Complex Biological Samples.
10:30 – 10:50
Michaela Scigelova
Thermo Fisher Scientific
Quantitation Approaches in Proteomics Using a Quadrupole-Orbitrap Mass Spectrometer
10:50 – 11:10
Stefan Bartram1, Sebastian Böcker3, Karin Groten1, Franziska Hufsky1,3, Alexander Muck2, Mariana Stanton1, Lynn Ullmann-Zeunert1, Natalie Wielsch1, Aleš Svatoš1
1 Max Planck Institute for Chemical Ecology, Jena, Germany
2 Waters GmbH, Eschborn, Germany
3 Friedrich Schiller University, Jena, Germany
Simultaneous determination of 15N-incorporation levels in plant proteins and their absolute quantitation to study nitrogen flux and protein turnover
11:10 – 11:30
Coffee / Refreshments / Trade Exhibition
11:30 – 13:20
Session 9: Structural and Functional Proteomics - Proteome Mining and Biomarkers
(Plenary Talks and selected talks)
Chair: Hana Kovářová, Suresh Jivan Gadher
 
11:30 – 12:15
Michael J. Taussig
Protein Technology Group, Babraham Bioscience Technologies, Cambridge, United Kingdom
Affinity Reagents and Protein Arrays for Proteome Analysis
12:15 – 13:00
Chris W. Turck
Max Planck Institute of Psychiatry, Munich, Germany
From proteomes to pathways to biosignatures – progress towards understanding psychiatric disorder etiology
13:00 – 13:20
Ondrej Vit1, Petr Man2, Jana Pospislova1, Lucie Lorkova1 and Jiří Petrák1,3
1 Institute of Pathological Physiology, First Faculty of Medicine, Charles University
2 Laboratory of Molecular Structure Characterization, Institute of Microbiology, v.v.i., Academy of Sciences of the Czech Republic,
3 Institute of Hematology and Blood Transfusion, Prague, Czech Republic
Beauty hidden in lipids. Proteomics of membrane proteins based on their transmembrane domains.
13:20 – 14:15
Lunch
14:15 – 15:30
Closing
(Karel Bezouška, Suresh Jivan Gadher, Hana Kovářová)
Chair: Karel Bezouška
 
14:15 – 15:00
Closing lecture
Vladimir Havlicek
Institute of Microbiology, Academy of Sciences of the Czech Republic, v.v.i., Vídeňská 1083, 142 20 Prague 4, Czech Republic
Mass spectrometry… Quo vadis?
15:00 – 15:30
Awards and Closing Remarks (Karel Bezouška, Suresh Jivan Gadher, Hana Kovářová)

MEETING CLOSED